A display substrate has a display area including a plurality of sub-display areas. The display substrate includes a base, a plurality of columns of pixels, a plurality of first and second power lines disposed on the base. At least two columns of pixels are disposed in each sub-display area, and each column of pixels includes at least three columns of sub-pixels. At least one first power line is disposed in each sub-display area and configured to provide a first power signal to all sub-pixels in a sub-display area. The second power lines are configured to provide second power signals to all sub-pixels. A plurality of columns of sub-pixels are disposed between any two adjacent power lines, and any first and second power lines are each arranged between two adjacent columns of sub-pixels emitting light of a first color and sub-pixels emitting light of a second color.