The present disclosure provides systems and methods for collecting multispectral images with a mobile device having a camera with an image sensor, such as a mobile phone. The systems and methods of the present disclosure control, support and power one or more light sources to emit single or multiple wavelengths of light onto an object. The system further supports and controls the camera within the mobile device to capture a series of images or video in the different or same light wavelengths and geometries. The resulting series of images may be used individually or collectively to detect and compare features in an object. This system may be used for the capture of multispectral images or video that could individually or collectively reveal features in the object that may not be visible in a single image.