A gas barrier film including a substrate film containing a resin, and a gas barrier layer that has oxygen barrier properties and is in contact with at least one surface of the substrate film, a surface of the gas barrier layer has a flat portion, and a plurality of protrusions that protrude from the flat portion. The number of protrusions that satisfy the following formula (1) is 0 to 200 per mm2, and the number of protrusions that satisfy the following formula (2) is 20 or more per mm2; d×2≤f(1); and 0.2≤f