- 专利标题: Method for producing a condensed adhesive phase of silk fusion proteins
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申请号: US16622301申请日: 2018-06-13
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公开(公告)号: US11597750B2公开(公告)日: 2023-03-07
- 发明人: Markus Linder , Pezhman Mohammadi , Sesilja Aranko
- 申请人: Aalto University Foundation sr
- 申请人地址: FI Aalto
- 专利权人: Aalto University Foundation sr
- 当前专利权人: Aalto University Foundation sr
- 当前专利权人地址: FI Aalto
- 代理机构: Laine IP Oy
- 优先权: FI20175544 20170613,FI20176165 20171222
- 国际申请: PCT/FI2018/050460 WO 20180613
- 国际公布: WO2018/229341 WO 20181220
- 主分类号: C07K14/435
- IPC分类号: C07K14/435 ; C07K1/02 ; C07K14/33 ; D01D1/02 ; D01F4/00 ; C12N15/62 ; A61L27/36
摘要:
The present invention is directed to a method for producing a condensed phase of a silk fusion protein, the method comprising the steps of preparing a solution of a silk fusion protein in an aqueous medium and concentrating the silk fusion protein in the aqueous medium, wherein the fusion protein is isolated from a recombinant production host and comprises a silk-like protein sequence and two separate non-silk terminal module sequences, such as cellulose binding modules, SpyCatcher domains, tenth type III module of Fibronectin, gamma-crystallin D, flanking the silk-like protein sequence; wherein the method is performed so that the silk fusion protein is not precipitated and subsequently dissolved to the aqueous medium. The present invention is also directed to using such fusion proteins as adhesives.
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