A patch and sensor assembly has a biosensor housed in a reusable portion that connects to a mapping and localization system (MLS) via biosensor wires. A disposable portion has an electrode layer through which signals are transmitted to the MLS via ACL wires. The biosensor and ACL wires extend through a single cable exiting the reusable portion. The disposable portion includes an adhesive layer for adhering the electrode layer to a patient, on the electrode layer, and an engagement element for detachably receiving at least a portion of the housing of the reusable portion. The reusable portion includes a housing in which the biosensor has biosensor wires that exit the reusable portion. The housing is also adapted to carry and/or provide support to an ACL contact member responsive to the electrode layer of the disposable portion, and ACL wires that exit the reusable portion.