A display device, a display panel, and a manufacturing method thereof are provided. The method includes: providing a display substrate including a display region having an opening region and an isolation region surrounding the opening region; forming a light-emitting layer covering the overall display region; forming a blocking layer on the light-emitting layer, the orthographic projection thereof on the display substrate covering an overall opening region and at least part of the isolation region; forming a first packaging layer covering the light-emitting layer and the blocking layer; forming a photoresist layer to pattern the first packaging layer and form a first through hole, the orthographic projection of the first through hole on the display substrate being within that of the blocking layer on the display substrate; removing the photoresist layer with stripper; and removing the blocking layer and part of the light-emitting layer corresponding to the first through hole.