A pixel compensation method, a pixel compensation device and a display device. The pixel compensation method includes: generating, according to grayscale data and compensation value of a sub-pixel in a current detection period, a source voltage signal to control light-emitting brightness of the sub-pixel; generating a sensing value according to the light-emitting brightness of the sub-pixel; and determining a compensation value of the sub-pixel in next detection period, according to the grayscale data, the compensation value, and the sensing value of the sub-pixel in the current detection period.