- 专利标题： Recombinant vector carrying cellulose binding domain and method for isolating and purifying protein, using same vector
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申请号： US16611966申请日： 2018-05-10
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公开(公告)号： US10934558B2公开(公告)日： 2021-03-02
- 发明人: Inhwan Hwang , Eun Ju Sohn , Yongjik Lee
- 申请人： BIOAPPLICATIONS INC.
- 申请人地址： KR Gyeongsangbuk-do
- 专利权人： BIOAPPLICATIONS INC.
- 当前专利权人： BIOAPPLICATIONS INC.
- 当前专利权人地址： KR Gyeongsangbuk-do
- 代理机构： Riverside Law LLP
- 优先权： KR10-2017-0058882 20170511
- 国际申请： PCT/KR2018/005371 WO 20180510
- 国际公布： WO2018/208099 WO 20181115
- 主分类号： C12N15/82
- IPC分类号： C12N15/82 ; C07K1/22 ; C07K1/30 ; C07K5/11 ; C07K14/195 ; C07K16/00 ; C07K14/35
摘要：
The present invention relates to a recombinant vector carrying cellulose-binding domain 3 and a method for separating a target protein using the recombinant vector. The protein isolating method of the present invention can easily separate a fusion protein containing a target protein by using the recombinant vector to bind a transformed plant body to cellulose and can effectively isolate the target protein from a cellulose-binding domain by treating the fusion protein with enterokinase, thus being expected to find industrial applications in various fields.
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