- 专利标题: Method for producing cerebellar progenitor tissue
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申请号: US15509387申请日: 2015-09-08
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公开(公告)号: US10781420B2公开(公告)日: 2020-09-22
- 发明人: Yoshiki Sasai , Keiko Muguruma
- 申请人: RIKEN , SUMITOMO CHEMICAL COMPANY, LIMITED
- 申请人地址: JP Wako JP Tokyo
- 专利权人: RIKEN,SUMITOMO CHEMICAL COMPANY, LIMITED
- 当前专利权人: RIKEN,SUMITOMO CHEMICAL COMPANY, LIMITED
- 当前专利权人地址: JP Wako JP Tokyo
- 代理机构: Leydig, Voit & Mayer, Ltd.
- 优先权: com.zzzhc.datahub.patent.etl.us.BibliographicData$PriorityClaim@28f3214
- 国际申请: PCT/JP2015/075412 WO 20150908
- 国际公布: WO2016/039317 WO 20160317
- 主分类号: C12N5/0793
- IPC分类号: C12N5/0793 ; C12N5/10 ; C12N15/09
摘要:
The present invention provides a method for producing a human cell aggregate containing a midbrain-hindbrain boundary neural progenitor tissue, including subjecting an aggregate of human pluripotent stem cells to suspension culturing in a serum-free medium containing insulin, and treating, in the suspension culturing, the aggregate of human pluripotent stem cells or a human cell aggregate derived therefrom with a ROCK inhibitor, a TGFβ signal inhibitor, and a first fibroblast growth factor. Furthermore, the human cell aggregate containing the midbrain-hindbrain boundary neural progenitor tissue is subjected to suspension culturing in a serum-free medium to induce formation of a neuroepithelial structure by neural progenitor in the neural progenitor tissue, whereby the human cell aggregate containing the cerebellar plate tissue can be obtained.
公开/授权文献
- US20170253853A1 METHOD FOR PRODUCING CEREBELLAR PROGENITOR TISSUE 公开/授权日:2017-09-07
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