Invention Grant
- Patent Title: Engineered polymerases for improved sequencing
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Application No.: US16567598Application Date: 2019-09-11
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Publication No.: US10731141B2Publication Date: 2020-08-04
- Inventor: Pinar Iyidogan
- Applicant: OMNIOME, INC.
- Applicant Address: US CA San Diego
- Assignee: OMNIOME, INC.
- Current Assignee: OMNIOME, INC.
- Current Assignee Address: US CA San Diego
- Agency: Kilpatrick Townsend & Stockton LLP
- Main IPC: C12N9/12
- IPC: C12N9/12 ; C12P19/34 ; C12Q1/6874

Abstract:
Provided are nucleic acids encoding engineered polymerases comprising at least one modification in a motif A and/or at least one modification in a motif B of the polymerase and engineered polymerases encoded by the nucleic acids. Also provided are engineered DNA polymerases comprising a variant of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, the variant being at least 80% identical to SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 and comprising an amino acid substitution at one or more positions selected from the group consisting of L408, Y409, P410, R484, A/L485, and I486. Methods, vectors, kits, and compositions comprising the nucleic acids and compositions, methods and kits comprising the engineered polymerases are also provided.
Public/Granted literature
- US20200087638A1 ENGINEERED POLYMERASES FOR IMPROVED SEQUENCING Public/Granted day:2020-03-19
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