An organic light-emitting diode, a manufacturing method thereof and a display device are provided. The organic light-emitting diode includes: a base substrate, a first electrode and a second electrode disposed on the base substrate, a first light-emitting layer disposed between the first electrode and the second electrode, and a charge generation layer disposed between the first light-emitting layer and the second electrode, and the charge generation layer includes a first connection layer, a carrier injection layer and a second connection layer which are stacked in sequence; the first connection layer includes an electron transport material doped with a hole blocking material, and the second connection layer includes a hole transport material doped with an electron blocking material.