The present disclosure provides an organic light-emitting diode and a method for manufacturing the same, a display substrate and a method for manufacturing the same, and a display device. The organic light-emitting diode comprises a first electrode, a second electrode, and an organic light-emitting layer arranged between the first electrode and the second electrode, in which the second electrode is arranged close to the light-emitting side of the organic light-emitting diode, the first electrode includes a reflective conductive layer, and a surface of the reflective conductive layer close to the second electrode has an uneven structure.