An imaging element includes microlenses, first pixel portions, and second pixel portions. The microlenses are arranged in first and second directions. The first and second pixel portions correspond to the microlenses. The first pixel portions include light receiving portions, which are pupil-split in the first direction. The second pixel portions include light receiving portions, which are pupil-split in the first direction. The light receiving portions include a light receiving region and a non-light receiving region along the second direction. The first pixel portions are arranged in the first direction and the second direction. The second pixel portions are substituted for some of the first pixel portions and are adjacently or discretely arranged in the second direction.