Embodiments are disclosed for emulation of graphics processing unit instructions. An example apparatus includes a kernel accessor to access an instruction of an original GPU kernel, the original GPU kernel intended to be executed at a first GPU. An instruction support determiner is to determine whether execution of the instruction is supported by a second GPU different from the first GPU. An instruction modifier is to, in response to determining that the execution of the instruction is not supported by the second GPU, create an instrumented GPU kernel based on the original GPU kernel. The instrumented GPU kernel includes an emulation sequence. The emulation sequence is to, when executed by the second GPU, cause the second GPU to emulate execution of the instruction by the first GPU.