It is disclosed a device and method for stripping a flexible substrate. The stripping device comprises: a platform for carrying a base plate on which a flexible substrate is formed; a pressing plate for being pressed onto the flexible substrate; and a laser generator for emitting laser for heating the base plate, so that the flexible substrate is decomposed on a side contacting the base plate and thus stripped; wherein the pressing plate is provided with a negative pressure passage, the pressing plate is provided with a suction mouth which is arranged in a side of the pressing plate facing the platform and which communicates with the negative pressure passage, and the negative pressure passage extracts through the suction mouth a gas which is generated during decomposition of the flexible substrate on a side contacting the base plate.