The utility model discloses a constant temperature inoculation jar, which comprises a tank body, jar body top is provided with the intake pipe, intake pipe one side is provided with the inlet pipe, inlet pipe middle part is provided with the non -return valve, and the top sets up the sealed lid, jar body is including a jar body bottom and jar can body that body middle part is constituteed, the can body has set gradually stainless steel skin, electromagnetic induction heating layer, heat preservation and inlayer outside from in to, heat preservation and inlayer are provided with the wall between 15, be provided with temperature detector in the wall 16, temperature detector and control by temperature change unit electric connection. In this way, constant temperature inoculation jar, utilize the intake pipe clean with the internal gaseous emission of jar during the inoculation, the vacuum keeps, guarantee that jar internal bacterial is pollution -free, purity is high, adopts the electromagnetic induction heating layer and heats heat preservation to the jar body, then guarantee through the control by temperature change unit that a jar internal portion is in the constant temperature state, is favorable to surviving of bacterial, the validity of assurance bacterial.