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发明授权
CN1970745B 脂肪酶的仿生亲和纯化方法 失效 - 权利终止 转让

脂肪酶的仿生亲和纯化方法
摘要：
本发明涉及一种脂肪酶的仿生亲和纯化方法，属于工业生物技术领域的。本发明包括下列步骤：(1)制备仿生亲和分离材料：首先用带氨基的基础层析介质与三氯三氮嗪进行反应，然后在50℃与烯丙胺反应，最后在95℃与环己胺反应，合成仿生亲和分离材料；(2)用仿生亲和分离材料纯化脂肪酶粗品：用上述制备的仿生亲和分离材料装入层析柱，得到亲和层析柱，随后将含脂肪酶的样品上至该亲和层析柱中，脂肪酶被柱中仿生亲和分离材料专一地吸附留在层析柱中，其它不能被吸附的蛋白随缓冲液流出，最后用洗脱缓冲液将脂肪酶从柱中专一洗脱下来，得到纯脂肪酶。本发明能够高效率、低成本的大规模生产高纯度的脂肪酶。
摘要（英）：

The invention discloses a bionic affiniting and purifying method in the industrial biological technical domain, which comprises the following steps: (1) reacting basic chromatographic dielectric withamino group and trichloazazine; reacting with allylamine at 50 deg.c; reacting with cyclohexylamine at 95 deg.c; synthesizing bionic affinity separating material; (2) purifying rough product of lipasethrough bionic affinity separating material; loading bionic affinity separating material in the chromatographic column; putting the lipase sample in the affinity chromatographic column; adorbing bonic affinity separating material in the chromatographic column; flowing non-adsorbed protein out slowly; eluting buffer from column to obtain pure lipase.

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