知搜-全球专利检索

  1. 登录
  2. 注册
发明授权
CN1916171B 一种生产木二糖的方法及其专用固定化酶 失效 - 权利终止

一种生产木二糖的方法及其专用固定化酶
摘要：
本发明公开了一种制备木二糖的方法及其专用固定化酶。本发明的固定化木聚糖酶按照包括如下步骤的方法制备：1)用金属螯合环氧载体Eupergit C 250L固定重组木聚糖酶XynB，得到固定化酶；2)将步骤1)得到的固定化酶悬浮于0.5-1.25M、pH为5.5-8.0磷酸盐缓冲液中，20-35℃反应12-60小时，得到固定化木聚糖酶；所述重组木聚糖酶XynB是将核苷酸序列是序列表中序列1的DNA序列的木聚糖酶基因经大肠杆菌表达得到的木聚糖酶。该固定化木聚糖酶的酶活力回收较高，稳定性和装柱后连续操作性能优良，便于连续化生产，且产物组成稳定易于控制；利用该固定化木聚糖酶生产木二糖，水解操作简单，可节约酶的用量，还可实现酶与水解液的分离，大幅度降低生产成本并提高生产效率。因此，该方法是一种适宜工业化生产木二糖的方法。
摘要（英）：

This invention discloses a method for preparing xylobiose and its specific immobilization enzyme. The method comprises: (1) immobilizing recombined xylanase XynB using metal-chelating epoxy carrier Eupergit C 250 L to obtain immobilization enzyme; (2) suspending the obtained immobilization enzyme in 0.5-1.25 M phosphate buffer with a pH of 5.5-8.0, and reacting at 20-35 deg.C for 12-60 h to obtained immobilization xylanase. The recombinant xylanase XynB is prepared by expressing xylanase gene (Sequence ID 1) in Escherichia coli. The obtained xylanase has such advantages as high recovery rate;high stability and good operation performance after loaded into the column, and can be used for continuous production. The method for preparing xylobiose by using xylanase is simple, and can save xylanase usage, realize separation of enzyme and hydrolyte, largely lower production cost and improve production efficiency, thus is suitable for mass production.

公开/授权文献
0/0