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发明授权
CN1896268B 从羊胚胎中提取活化小分子羊胎素的方法及其用途 有权 转让

从羊胚胎中提取活化小分子羊胎素的方法及其用途
摘要：
从羊胚胎中提取活化小分子羊胎素的方法及其用途，属生物工程技术领域，将去除肠胃肠的40～90日龄新鲜羊胚胎切碎后，加蒸馏水，制成匀浆，在-20℃条件下反复冻融，过滤，将滤液加热后，分别取得滤过液A及沉淀物A；将滤过液A超滤，取透明淡黄色滤过液B和沉淀物B；将沉淀物A及沉淀物B合并，调pH至2.0，加入胃蛋白酶，再调pH至7.0，在100±2℃条件下保温后，取滤过液C；将滤过液B和滤过液C合并，即为活化小分子羊胎素。可用于制备外用祛除皱纹、美化皮肤用品。本发明的羊胎素分子量控制在3000～6000之间，大部分为小分子肽及细胞因子成份，活化性高，减少了过敏等副作用，并提高了皮肤吸收率。
摘要（英）：

The extraction of active small molecular placentin from lamb embryo and its purpose that belongs to the bioengineering field includes the following steps: removing stomach and intestine from fresh lamb embryo of 40-90 days and cutting up, homogenizing with distilled water, frost thawing repeatedly at -20DEG C, filtrating, heating the filtrate and obtaining Filtrate A and Sediment A; ultra-filtrating Filtrate A and obtaining the transparent primrose yellow Filtrate B and Sediment B; mingling Sediment A and Sediment B, modifying pH to 2.0, adding pepsin, then modifying pH to 7.0, incubating at100 +-2DEG C and preserving Filtrate C; active small molecular placentin is obtained by mingling Filtrate B and C that can be used in the preparation of skin care product. The placentin prepared in this invention has the molecular weight of 3000-6000 and contains mainly cytokines and small peptides, has high activity, low side-effect such as anaphylactic response, and increased skin absorption ratio.

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