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发明授权
CN1807608B γ-生育酚甲基转移酶基因、其表达载体及应用 失效 - 权利终止

γ-生育酚甲基转移酶基因、其表达载体及应用
摘要：
本发明分别公开了源于玉米、大豆的γ-生育酚甲基转移酶、其编码基因及它们在植物基因工程领域中的用途。本发明采用RACE及RT-PCR技术，分别从油料作物高油玉米115、大豆(郑9525)中获得SEQ ID NO：1和SEQ IDNO：3所示的γ-生育酚甲基转移酶的全长cDNA编码序列，并通过原核表达获得了有生物活性的γ-生育酚甲基转移酶蛋白，同时还比较了不同来源的γ-TMT酶比活的差异。本发明分别将从玉米、大豆中分离的γ-生育酚甲基转移酶cDNA编码序列转化模式植物，经HPLC分析转基因植物叶子中的α-生育酚的含量比野生对照约提高2～5倍。本发明为培育高维生素E含量的油料作物新品种开辟了一条新途径。
摘要（英）：

This invention discloses a gamma- tocopherol methyl transfer enzyme that from corn and soybean, their code gene and their use in the plant gene project field. This invention uses RACE and RT-PCR technique, it obtains the span cDNA code sequence of gamma- tocopherol methyl transfer enzyme showed by the SEQ ID NO: 1 and SEQ ID NO: 3 from the corn of high oil 115 and soybean (9525) which all belongsto the oil plants, and it obtains the gamma- tocopherol methyl transfer enzyme with biological activity through prokaryotes expression, and it also compares with the difference of gamma-TMT enzyme activity form different sources. This invention transforms the pattern plant from the related code sequence, through HPLC analysis, the content of the alpha- tocopherol of the transfer gene plant leafage is improved by 2-5 times comparing to that of the wild leafage. This invention is to cut a new way for culturing the new plant of oil plants with high vitamin E content.

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