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发明授权
CN1694953B 制备哺乳动物胰蛋白酶的方法 失效 - 权利终止

制备哺乳动物胰蛋白酶的方法
摘要：
本发明涉及在Fusarium venenatum宿主菌株中制备哺乳动物胰蛋白酶的方法，包括(a)在适宜表达所述哺乳动物胰蛋白酶并将其分泌到培养基中的条件下在培养基中培养所述Fusarium venenatum宿主菌株，其中所述Fusarium venenatum宿主菌株包含含有核酸序列的核酸构建体，所述核酸序列编码哺乳动物胰蛋白酶的成熟编码序列并与编码尖镰孢胰蛋白酶原的信号肽和原肽的SEQ ID NO：1的核苷酸58-129可操作连接；和(b)从所述培养基中回收所述哺乳动物胰蛋白酶。本发明还涉及包含核酸序列的构建体，所述核酸序列编码哺乳动物胰蛋白酶的成熟编码序列、并与编码尖镰孢胰蛋白酶原的信号肽和原肽的SEQ ID NO：1的核苷酸58-129可操作地连接，以及涉及包含所述构建体的载体和Fusarium venenatum宿主菌株。
摘要（英）：

The present invention relates to methods for producing a mammalian trypsin in a Fusarium venenatum host strain, comprising (a) cultivating the Fusarium venenatum host strain in a culture medium underconditions suitable for expression of the mammalian trypsin and secretion thereof into the medium, wherein the Fusarium venenatum host strain comprises a nucleic acid construct comprising a nucleic acid sequence encoding the mature coding sequence of a mammalian trypsin operably linked to nucleotides 58 to 129 of SEQ ID NO: 1 encoding the signal peptide and propeptide of Fusarium oxysporum trypsinogen; and (b) recovering the mammalian trypsin from the medium. The present invention also relates to constructs comprising a nucleic acid sequence encoding the mature coding sequence of a mammalian trypsin operably linked to nucleotides 58 to 129 of SEQ ID NO: 1 encoding the signal peptide and propeptide of Fusarium oxysporum trypsinogen, and to vectors and Fusarium venenatum host strains comprising such constructs.

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