The invention discloses an immune chromatography test paper based on luminous technique with upside conversion. Use UCP as biological mark, show the result with format of visible light signal on the exposure of infrared light, interpret the result by device, then realize the quantitative detection to object. The structure main comprises sample pad, combination pad or conjugate pad, analysis membrane, sopping pad and plastic backboard. The invention also discloses the preparation and application of the test paper in biological sample quantitative detection. According to the difference of immunological reaction ways of material, classify the test paper to sandwich mode, competition mode and indirect mode; all modes can take qualitative and quantitative detection to different material quicklyand acutely.