The present invention discloses one method of growing cubic texture nickel oxide isolation layer. By means of simple operation process, cubic texture nickel oxide isolation layer is grown through oxidizing epitaxy on the surface of cubic texture metal nickel. Specifically, the cubic texture metal nickel sheet is cleaned and oxidized in air environment under 1100-1250 deg.c for 3-30 min. Highly cubic texture nickel layer is obtained on the surface of cubic texture metal nickel and used mainly as the isolating layer for high temperature superconductive coating conductor.