The invention discloses a mouse embryonic stem cell (ESC) induction culture solution for improving 2C-like cells and a mouse ESC induction culture method. The method comprises the steps that: first, fetal bovine serum is removed from a mouse ESCs culture system, so that mouse ESCs still maintain original pluripotency characteristics; and second, retinoic acid (RA) is utilized for increasing the proportion of the 2C-like cells in the mouse ESCs in a culture solution without containing the fetal bovine serum, and the result is increased from 0.2% of a control group to 3.3%. The 2C-like cell proportion in the mouse ESCs is increased by using a specific induction method, the 2C-like gene expression level and protein level of the mouse ESCs in the culture solution without containing the fetal bovine serum are verified by experimental means, and a new thought is provided for research of mammal totipotent stem cells.