The present invention discloses a preparation method of an extremely fresh composite seasoning material based on evaluation of seasoning material freshness, and belongs to the field of food processing. Pleurotus eryngii powder, flammulina velutipe powder, soft-shelled turtle waste powder, shrimp meat powder and gluten powder are used as raw materials, a slow freezing and freeze-drying, twin-screwextrusion, ultrasonic and bio-enzymatic mixing technology is used, the extremely fresh composite seasoning material is prepared, an application of the mixing technology shortens preparation time of aconventional biological enzymolysis technology, improves production efficiency, enhances product delicate flavor and flavor, and improves product quality. A commonly used flavor enhancer sodium glutamate is used as a standard sample to manufacture a delicate flavor intensity classification standard, an electronic tongue detection is combined with sensory evaluation to detect delicate flavor values, a slight delicate grade is determined when delicate flavor value is smaller than 0.3% sodium glutamate concentration delicate flavor value, a delicate grade is determined when the delicate flavor value is between 0.3-0.6% sodium glutamate concentration delicate flavor value, a strong delicate grade is determined when the delicate flavor value is between 0.6-0.8% sodium glutamate concentration delicate flavor, and an extreme delicate grade is determined when the delicate flavor value is larger than 0.8%.