The invention relates to the field of medical immunology, in particular to a kit for detecting a mutant citrullinated vimentin antibody. The kit comprises a sample diluent, magnetic particles coupledwith an MCV antigen, an enzyme conjugate and a luminescent substrate; the enzyme conjugate is prepared from an enzyme-labeled mouse-anti-human IgG antibody and an enzyme conjugate diluent; and the sample diluent is a 0.05M phosphate buffer solution which has the pH of 7.4 and is prepared from 0.5%-2% of bovine serum albumin, 0.5%-1% of Casein protein, a heterophil antibody blocker with the concentration of 100-500 ng/ml and 0.1%-5% of a metal chelator. The kit is high in sensitivity, good in specificity, wide in linear range, good in stability and convenient to use. In addition, the kit can beequipped with an automatic system and is easy to operate and high in flux; and a magnetic particle platform is adopted, the application range is wide, and the cost is low.