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发明公开
CN110106160A 催化活性提高的碱性蛋白酶突变体PROK-G及其编码基因和应用 有权 转让

催化活性提高的碱性蛋白酶突变体PROK-G及其编码基因和应用
摘要：
本发明属于农业生物技术领域，具体涉及催化活性提高的碱性蛋白酶突变体PROK-G及其编码基因和应用。本发明以Parengyodontium album来源的碱性蛋白酶PROK作为母本，经点突变His208Phe/Thr212Asn，而获得的碱性蛋白酶PROK突变体。本发明获得碱性蛋白酶突变体的催化活性比野生型明显提高。
摘要（英）：

The invention belongs to the technical field of agricultural biology, and particularly relates to an alkaline protease mutant PROK-G with the improved catalytic activity and an encoding gene and application thereof. The alkaline protease PROK from Parengyodontium album is adopted as a female parent, and due to point mutation His208Phe/Thr212Asn, the alkaline protease PROK mutant is obtained. The catalytic activity of the obtained alkaline protease mutant is improved obviously compared with a wild type.

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