The invention provides a preparation method of a close-packed thin film based on a two-dimensional quantum chip. The preparation method mainly comprises the following steps: sucking-filtering grapheneoxide suspension on a surface of a filter membrane so as to form a layer of graphene oxide membrane, adding two-dimensional quantum chip suspension on the surface of the graphene oxide membrane, andthen sucking-filtering to form a membrane; immersing the sucked-filtered composite membrane into the water, performing separation, and then obtaining a complete two-dimensional quantum chip close-packed thin film. The two-dimensional quantum chip close-packed thin film obtained through the preparation method provided by the invention is adjustable in thickness and size; on a microstructure, a structure similar to the brick type is formed by alternately and closely packing the two-dimensional quantum chips in an angle approaching to the horizontal angle. The preparation method provided by the invention is simple in process, fast and efficient, green and environmentally friendly, and a raw material is provided for searching the basic physical property and innovation application of the close-packed thin film based on the two-dimensional quantum chip.