The invention belongs to technical field of seasonings, and particularly relates to seafood seasoning sauce rich in protein and amino acid and a preparation method of the seafood seasoning sauce. Theseafood seasoning sauce comprises, by weight, 20-30 parts of soybeans, 20-30 parts of black soybeans, 20-25 parts of squid bones, 20-30 parts scallop meat, 10-20 parts of dried small shrimps, 10-15 parts of seaweed gel, 5-10 parts of Chinese magnoliavine fruits and 5-10 parts of barbary wolfberry fruits, 5-10 parts of Japanese cinnamon barks, 10-15 parts of common ginger rhizomes and 20-30 parts of salt. The seasoning sauce prepared by the method is delicious in taste and balanced in nutrition, and the preparation method is simple in process and low in cost.