The invention discloses a method for preparing (R)-m-nitrobenzene glycol by using bean bean epoxide hydrolase, which belongs to the technical field of biological catalysis. The method uses a whole cell of Escherichia coli expressing epoxide hydrolase PvEH2 as a catalyst, according to the excellent stereoselective property against racemic m-nitroxyphenylethane, by establishing a two-phase system-petroleum ether/water, the initial loading concentration of a substrate and the efficiency of an entire catalytic reaction are improved, and inhibition of the high-concentration m-nitroepoxybenzene in asingle aqueous phase system for PvEH2 activity is alleviated, at the same time, the degree of spontaneous hydrolysis of the substrate is reduced, the enantiomeric purity (eep) of the product vicinaldiol is up to 90.2%, and the conversion rate of the substrate is up to 99.5%.