The invention relates to the technical field of luminous compounds, in particular to an organic electroluminescence compound and a preparation method and application thereof. In order to obtain an ideal material to solve the problems existing in a traditional hole-transport material, the solution that arylamine and an anthracene derivative are introduced to the 9-position of fluorene is provided.By introducing the arylamine, the hole injection ability, the hole transfer ability, the high power efficiency and long life are obtained; by introducing the anthracene derivative, a proper glass transition temperature is obtained, and therefore, the high-quality organic electroluminescence compound is obtained. A device prepared from the compound is excellent in current efficiency and power efficiency and long in life. The preparation method of the organic electroluminescence compound is simple and easy to implement, high in yield and suitable for industrial production.