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发明授权
CN109735520B 一种具有热稳定特性的ι-卡拉胶酶及其应用 有权

一种具有热稳定特性的ι-卡拉胶酶及其应用
摘要：
本发明涉及一种具有热稳定特性的ι‑卡拉胶酶CgiV及其应用。本发明提供的ι‑卡拉胶酶基因来源于海洋细菌Vibrio sp.SY01，菌株保藏于中国典型培养物保藏中心，保藏号CCTCC No：M2018769。所述ι‑卡拉胶酶CgiV的氨基酸序列如SEQ ID NO.1所示。氨基酸序列与已知功能的ι‑卡拉胶酶相似度仅为88％。本发明所述的ι‑卡拉胶酶为重组表达工程菌株纯化所得，具有热稳定特性，在40℃的活性半衰期长达6.5h，30℃的活性半衰期长达42h，具备工业化应用潜质。
摘要（英）：

The invention relates to an l-carrageenan enzyme CgiV with thermal stability and application thereof. A provided l-carrageenan enzyme gene is derived from marine bacteria Vibrio sp.SY01, a strain is preserved in the China's typical culture collection center, and the preservation number of the strain is CCTCC No: M2018769. The amino acid sequence of the l-carrageenan enzyme CgiV is shown as SEQ IDNO.1. The similarity between the amino acid sequence of the l-carrageenan enzyme CgiV and the amino acid sequence of an l-carrageenan enzyme with known functions is only 88%. The l-carrageenan enzymeis obtained through purification of recombinant expression engineering strains and has thermal stability, the activity half-life of the l-carrageenan enzyme at 40 DEG C is 6.5 hours, and the activityhalf-life of the l-carrageenan enzyme at 30 DEG C is 42 hours. The l-carrageenan enzyme has industrial application potential.

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