The invention discloses a production method for a high-activity yolk fermented substance. The production method comprises the following steps: 1) breaking egg shells and separating, thereby acquiringa yolk fluid, and then adding wood vinegar, soaking, stirring and performing enzyme hydrolysis, thereby acquiring a hydrolyzed yolk fluid; 2) heating the hydrolyzed yolk fluid in water bath, adding gelatin and catechinic acid, stirring under a constant temperature, and inoculating yeast and lactic acid bacteria, thereby acquiring a fermented yolk fluid; 3) filtering and homogenizing the fermentedyolk fluid, regulating pH, heating, preserving heat, centrifugally separating, standing by and layering, removing superstratum grease and bottom precipitation, freezing, unfreezing, heating in water bath, adding cyclodextrin under an ultrasonic-assisted condition, quickly dispersing, mixing, embedding, cooling under low temperature and standing by, centrifugally removing cholesterol, and freeze-drying the separated fluid, thereby acquiring a lecithin polypeptide-rich low cholesterol yolk fermented substance. The invention also discloses the high-activity yolk fermented substance and an application thereof. The extract according to the invention has higher biological activity and is capable of achieving positive influence on basic metabolism and immune system of human body.