The invention discloses a (+)-2,6-bis(4,5-pinene-2-pyridyl)pyridine chiral mononuclear manganese complex and a preparation method thereof. A molecular formula of the complex is Mn(L)2.2ClO4. The preparation method comprises following steps of adding an acetonitrile solution in which Mn(ClO4)2.6H2O is dissolved to a dichloromethane solution in which a chiral tridentate nitrogen-containing organic ligand L is dissolved, constantly stirring for 15-20 minutes and then filtering, standing to volatilize an obtained clear solution, 2-3 days later, obtaining yellow block crystal, filtering, washing with water, and vacuum drying so as to obtain the (+)-2,6-bis(4,5-pinene-2-pyridyl)pyridine chiral mononuclear manganese complex. The chiral mononuclear manganese complex is synthesized under mild conditions, has high yield, has strong stability, has high reproducibility, is easy to separate and purify, and has a broad application prospect in chiral asymmetric catalysis, chiral enantiomeric separation, chiral induction and so on.