The invention provides a remote operation guidance method and system. The method comprises that a smart terminal adds a first relative time stamp to an acquired audio, a video and AR annotation information to synchronize the three, and then sends the synchronized three to a WEB terminal; the smart terminal acquires a video and remote assistance information added with second relative time stamps and returned by the WEB terminal; the smart terminal synchronously replays the audio, the video and the AR annotation information according to the first relative time stamp, and simultaneously synchronously replays the video and the remote assistance information according to the second relative time stamp; the smart terminal uses the video as a mainstream to display the video, the audio, the AR annotation information and the remote assistance information which are synchronously replays. The technical solution provided by the invention realizes the synchronization of the audio, the video and AR annotation information by using the relative time stamp synchronization method, realizes the synchronous display of the video, the audio, the AR annotation information and the remote assistance information by using the synchronous replay, and achieves precise remote guidance.