The invention discloses an anti-tumor protein and an application thereof, belonging to the technical field of biomedicine. The invention firstly screens and verifies the application of PvTrag17 protein shown in SEQ ID NO. 1 in anti-tumor field, The results of CCK8 proliferation assay showed that PvTrag17 protein inhibited the proliferation of HepG2 cells by 63.1% and almost completely inhibited the migration of HepG2 cells. The PvTrag17 protein of the invention can be used as an antineoplastic drug of active ingredient or raw material, and has wide application prospect.