The invention discloses a preparation method of selenium-enriched lemon wine, and relates to the technical field of preparation of fruit wine. The method comprises the steps of culture preparation, lemon pretreatment, fermentation, ageing and canning. The method solves the problems that common yeast is difficult to survive and proliferate in a lemon high acid environment, the culture activity is low and the wine productive rate is low by inducing yeast to produce acid, domesticate the yeast to adapt to acid environment, and applying the yeast to lemon wine fermentation, guarantees the normal growth and proliferation of the yeast, and improves the wine yield, and meanwhile the lemon wine is rich in selenium element, and achieves a standard of selenium-enriched food.