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发明公开
CN108823224A 一种高表达的耐热酸性木聚糖酶基因及其表达载体和应用 无效 - 撤回

一种高表达的耐热酸性木聚糖酶基因及其表达载体和应用
摘要：
本发明公开了一种高表达的耐热酸性木聚糖酶基因及其表达载体和应用。对来源于黑曲霉(Aspergillus niger)的耐热酸性木聚糖酶基因XynZN进行改造后得到适宜在毕赤酵母中表达的优化耐热酸性木聚糖酶基因XynZNOp。本发明进一步构建了重组毕赤酵母组成型和诱导型载体并将其转化到毕赤酵母中筛选得到高效表达耐热酸性木聚糖酶的重组菌株，结果显示在重组菌株中XynZNOp基因相对于野生型的XynZN基因表达量有着显著提高，在组成型和诱导型重组菌株中提高幅度分别为52.0％和46.3％。使用本发明耐热酸性木聚糖酶XynZNOp优化基因序列构建的毕赤酵母重组菌株，目的蛋白表达量显著提高，极大的降低了耐热酸性木聚糖酶的生产成本，为其工业化扩大生产应用奠定了基础。
摘要（英）：

The invention discloses a high-expression heat-resisting acidic xylanase gene as well as an expression vector and an application thereof. After a heat-resisting acidic xylanase gene XynZN derived fromAspergillus niger is modified, an optimized heat-resisting acidic xylanase gene XynZNOp suitable for being expressed in pichia pastoris is obtained. Recombinant pichia pastoris constitutive and induced vectors are further constructed and transformed into pichia pastoris and recombinant strains capable of efficiently expressing heat-resisting acidic xylanase are screened out, a result shows that the expression quantity of the XynZNOp gene in the recombinant strains is remarkably increased relative to the expression quantity of a wild type XynZN gene, and increased ranges of the expression quantity in the constitutive and induced recombinant strains are 52.0% and 46.3% respectively. According to pichia pastoris recombinant strains constructed by adopting a heat-resisting acidic xylanase XynZNOp optimized gene sequence, target protein expression is remarkably improved, production cost of heat-resisting acidic xylanase is greatly reduced, and a foundation is laid for industrialized expansion of production application.

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