The invention belongs to the technical field of seedling cultivation of forest trees and provides a method for cutting, rapid propagation and seedling cultivation of tender buds of seabuckthorn in order to overcome the defects that during current cutting of hard branches and tender branches, cuttings are difficult to obtain and the cost for manually obtaining the cuttings is high. A micro-cuttagetechnology with the tender buds of the seabuckthorn as the cuttings can be directly used in a cutting and rapid propagation system of the tender buds of the seabuckthorn for production. Thousands of buds serving as the cuttings are taken from a perennial parental seabuckthorn plant with excellent properties at the same time, and roots can grow in 7-15 days. By adopting a treatment method of usingIBA, potassium permanganate and aspirin, the survival rate of the roots is increased by 20% in comparison with a method of only using hormones for treatment of the tender buds. A used substrate preferably contains vermiculite and river sand with the proportion of 1:1, nutrient soil is not added, and 0.1% potassium permanganate is used for soaking and disinfection first, so that mildewing before the tender buds are rooted is effectively controlled. Through establishment of the seabuckthorn asexual seedling cultivation system, propagation of the excellent seabuckthorn parental plant can be greatly expanded in a short time, and high practicability is achieved. In addition, the operation is simple, the cost is low, and the method can be used for production and is easy to popularize.