The invention discloses a Scopulariopsis brevicaulis strain WNF22 and application thereof. The Scopulariopsis brevicaulis strain WNF22 is preserved in China Center for Type Culture Collection on May 21, 2018, with an accession number of CCTCC No. M 2018293. The Scopulariopsis brevicaulis strain WNF22 is a bacterial strain capable of highly efficiently degrading sulfonated brown coal and waste sulfonated water-based drilling fluids, and has good stress resistance. When the strain WNF22 is combined with plants for treatment of waste sulfonated water-based drilling fluids, good removal effect isexerted on the COD, total petroleum hydrocarbon (TPH) and sulfides in the leachate of samples. The strain can be used as an excellent bacterial strain for biological treatment of waste sulfonated water-based drilling fluids and has good application prospects.