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发明授权
CN108753741B 一种胞外AA9家族多糖单加氧酶AnLPMO15g及其应用 有权

一种胞外AA9家族多糖单加氧酶AnLPMO15g及其应用
摘要：
本发明提供了一种胞外AA9家族多糖单加氧酶AnLPMO15g及其应用，所述胞外AA9家族多糖单加氧酶AnLPMO15g的核苷酸序列如SEQ ID NO.1所示。本发明所述的AnLPMO15g可单独作用于纤维素类底物，对其糖苷键进行C1位氧化切割产生还原糖，该酶与商品纤维素酶协同作用，可分别将商品纤维素酶诺维信Ctec2降解微晶纤维素和草粉的还原糖产量提高96.75％和131.55％。
摘要（英）：

The present invention provides an extracellular AA9 family polysaccharide monooxygenase AnLPMO15g and applications thereof, wherein the nucleotide sequence of the extracellular AA9 family polysaccharide monooxygenase AnLPMO15g is represented by SEQ ID NO.1. According to the present invention, the AnLPMO 15g can separately act on cellulose-based substrates, wherein the glycosidic bond is subjectedto C1 site oxidative cleave to produce the reducing sugar; and through the synergetic effect of the extracellular AA9 family polysaccharide monooxygenase AnLPMO15g and the commercial cellulase, the yields of the reducing sugars in the microcrystalline cellulose degradation and the grass powder degradation by the commercial cellulase Novozyme Ctec2 can be respectively increased by 96.75% and 131.55%.

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