The invention belongs to a positioning device for a networking measuring instrument and a preparation and use method thereof. The positioning device is composed of an instrument fixing bolt, and a through hole which can be inserted with a concrete stand column in a sleeving mode is formed in the middle of a bottom ring plate; the bottom ends of at least three brackets are fixed to the bottom ringplate on the outer side of the through hole at intervals, and the top ends of the brackets are fixed to the side edge portions of the corresponding faces of a top steel plate at intervals; and bracketnut holes are breadthwise formed in the brackets on the lower portion of a stand column fixing ring, T-shaped screws are arranged in the bracket nut holes in a threaded mode, an adjusting hole is formed in the center of the top steel plate, and a strip-shaped hole is formed in the middle of an adjusting strip plate; and the instrument fixing bolt is located in the strip-shaped hole in the adjusting hole. The preparation and use method includes the steps of installation, leveling and centring of the positioning device. The positioning device can improve the deficiency of existing installationmethods, and has the advantages of firm installation, good stability, easy adjustment, time saving, labor saving and good use effect.