The invention belongs to the technical field of food processing and discloses gingko bread and a preparation method thereof. The gingko bread is prepared from, by weight, 35-45 parts of gingko detoxifying powder, 200-230 parts of wheat flour, 50-75 parts of multigrain flour, 50-70 parts of honey, 50-70 parts of eggs, 100-120 parts of milk, 30-50 parts of butter, 20-25 parts of cheese, 4-6 parts ofsalt, an appropriate amount of water, chocolates, nuts, ice creams, pigskin, fresh meat and lemon juice. The preparation method of the gingko bread includes steps of raw material preparation, stuffing preparation, chocolate ball preparation, raw material mixing, fermenting, baking, filling, freezing, packaging for storing and edible method. By adding of gingko nuts, nutrients of the bread are increased; by adding of the stuffing and the chocolate balls, nutrient elements of the bread are increased while bread flavor is improved, taste and layered palatability of the bread are enriched, and the bread is unique in flavor and high in edible value.