The invention discloses polyester based on a full biomass monomer, a preparation method and an application, and belongs to the field of polyester synthesis. Polyester takes 2,5-dimethoxy dimethyl terephthalate with a cyclic rigid group, and isosorbitol as raw materials, obtaining an ester exchange product through a catalytical ester exchange reaction, obtaining a polyester crude product through acatalytical polycondensation reaction, and performing solvent extraction, precipitant precipitation, filtration and drying to form target polyester. Synthesized polyester has the characteristics of high molecular weight, high heat performance, good mechanical properties and the like, and the characteristic of easy degradation in a natural soil environment as biobased polylactic acid. Polyester based on the full biomass monomer is mainly applied to preparing a bottle level polyester material.