The invention discloses a wire confocal imaging device for multichannel scanning and detecting, which comprises an exciting light module, a scanning imaging module and a detecting module. The excitinglight module is used for providing two separated linear beam patterns obtained through shaping two laser beams; the inclination angles and the divergence angles of the laser beams are adjustable; thescanning imaging module is used for focusing the two linear beam patterns to different imaging positions, so that two imaging light beams are produced, and three-dimensional scanning imaging is realized; the detecting module is used for separating and detecting the two imaging light beams; through fine adjusting the inclination angles and the divergence angles of the two laser beams in the exciting light module, simultaneous scanning image of double focal planes or simultaneous scanning imaging of double lines in the same focal plane of a simple objective angular field can be realized. According to the wire confocal imaging device for multichannel scanning and detecting provided by the invention, the imaging speed can be effectively improved, and meanwhile, the system adjustment can be carried out conveniently.