The present invention relates to new uses of daphnetin in inhibition of Helicobacter pylori. According to the present invention, the daphnetin can well kill a variety of drug-resistant phenotype Helicobacter pylori; particularly the results show that the daphnetin can produce the zone-of-inhibition with a diameter of 39-53 mm at a concentration of 100 [mu]g/disc, and has the minimum inhibitory concentration (MIC) of 25-100 [mu]g/ml; and the new use of the daphnetin provide the new option for Helicobacter pylori inhibition drugs.