Provided are a Tripterygium wilfordii Cryptomeridiol synthase and a coding gene thereof. Also provided are a Tripterygium wilfordii Cryptomeridiol synthase and a coding gene, a engineered yeast contianing the Tripterygium wilfordii Cryptomeridiol coding gene, and a use of same in plant breeding and biosynthesis. The cDNA full-length sequence of the Tripterygium wilfordii Cryptomeridiol synthase gene in Tripterygium wilfordii is obtained by means of polymerase chain reaction cloning. Then, by means of synthetic biology, the engineered yeast containing the Tripterygium wilfordii Cryptomeridiol synthase gene is constructed to realize the production of Tripterygium wilfordii Cryptomeridiol in the yeast.