The invention provides a spectral line lamp for spectrometer wavelength calibration. The spectral lien lamp comprises a lamp housing and two electrodes, wherein the lamp housing is filled with mercury-neon mixed gas. According to the spectral line lamp for spectrometer wavelength calibration, through filling the housing with the mercury-neon mixed gas, after an AC high-voltage pulse is applied tothe spectral line lamp, a plurality of line spectrums can be generated in a 250nm-2500nm waveband range. The spectral line lamp has advantages of simple structure, wide application range and high practicability.