知搜-全球专利检索

  1. 登录
  2. 注册
发明公开
CN108048600A 一种牛传染性鼻气管炎病毒的荧光定量PCR检测方法 有权

一种牛传染性鼻气管炎病毒的荧光定量PCR检测方法
摘要：
本发明公开了一种荧光定量PCR检测牛传染性鼻气管炎病毒(IBRV)的gE和gB基因的引物、探针、试剂盒及方法，所述方法为以待检测样品的DNA为模板，进行荧光定量PCR扩增反应，收集荧光信号，绘制曲线。本发明的检测牛传染性鼻气管炎病毒(IBRV)的引物和探针具有很高的特异性和灵敏度，能够检测出牛传染性鼻气管炎病毒(IBRV)，可以对各种临床样品进行检测，从而可以快捷的对临床中牛传染性鼻气管炎病毒(IBRV)感染情况进行监测，检测时间短，检测灵敏度高，稳定性高。
摘要（英）：

The invention discloses primers, probes, a kit and a method for detecting the genes gE and gB of infectious bovine rhinotracheitis virus (IBRV) through fluorescence quantitative PCR. According to themethod, the DNA of a sample to be detected is used as a template, a fluorescence quantitative PCR amplification reaction is performed, fluorescence signals are collected, and a curve is drawn. According to the present invention, the primers and the probe have high specificity and high sensitivity, can detect infectious bovine rhinotracheitis virus (IBRV), and can detect various clinical samples soas to quickly monitor the infectious bovine rhinotracheitis virus (IBRV) infection condition in clinic, such that the detection time is short, the detection sensitivity is high, and the stability ishigh.

公开/授权文献
0/0