The invention relates to the field of chemical and industrial machine, and particularly discloses a high-speed adverse current chromatographic instrument under intensified effect of the electric field. The high-speed adverse current chromatographic instrument comprises a base, a driving device, a rotating device, an electric field device, and at least two groups of separating units. Solution in aseparating pipe is cooled through placing the separating pipe with small pipe diameter in the water pipe with big diameter, and continuously filling circular cooling fluid in the cooling water pipe totake away the joule heat generated during the electrifyingprocess of the separating pipe. The high-speed adverse current chromatographic instrument under intensified effect of electric field can wellfuse the high-speed adverse current chromatographic separating technology with an electric promoting split-phase technology; two high-efficient separating technologies are used for performing the separating test in the same device while displaying the advantages of the high-speed adverse current chromatographic separating technology and the electric promoting split-phase technology; thus fluid inthe separating pipe is cooled, the bioactivity of the separating sample is kept, the separating efficiency is greatly improved, and the separating time is shortened; besides, the high-speed adverse current chromatographic instrument is strong in adaptability, high in separating efficiency, wide in application range, and others.